· possible to adjust the system to enable synchronized bead delivery. Such a modification could significantly improve screening efficiency and throughput. In conclusion, we have presented a screen
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· These biosensor modifications increased the high-throughput screening efficiency of yeast cells engineered to produce PHBA by 5,000-fold, enabling accurate fluorescence activated cell sorting
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:Fredrik Svensson, Ulf Norinder, Ulf Norinder, Andreas BenderPublish Year:2017 · Here, we discuss about various aspects of three representative high-throughput screening (HTS) systems: microwell-, droplet-, and single-cell-based
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:High Throughput ScreeningPublish Year:2012 · Screening capacity can be described by the following formula with factors specified below: Qu = A x B x C x D x E x F x G x H x J x K x L x Af, where: Qu = Screening capacity. [t/h] (passing through)
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:High-Throughput TechnologyHTS · High-throughput screening (HTS) facilitates selection of the best performing strain. •. Biosensor-enabled HTS obviates the need for sophisticated
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· Screening large pools of molecular candidates to identify those with specific design criteria or targeted properties is demanding in various science and engineering domains. While a high-throughput virtual screening (HTVS) pipeline can provide efficient means to achieving this goal, its design and operation often rely on
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ML for genomic tumor marker screening. 3.1. Application of ML for screening markers derived from circulating tumor DNA (ctDNA), cell-free DNA (cfDNA), and DNA methylation data. High-throughput genomic data often have high dimensionality and complexity, and therefore require preprocessing to reduce noise and redundancy.
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· These biosensor modifications increased the high-throughput screening efficiency of yeast cells engineered to produce PHBA by 5,000-fold, enabling accurate fluorescence activated cell sorting isolation of producer cells
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· These results indicate that iterative screening based on molecular docking and conformal prediction can be an efficient way to find active compounds while screening only a small part of the compound collection.
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· The rapid testing of chemical libraries for biological activity is the primary aim of high throughput screening (HTS). Advances in HTS have paralleled those in molecular biology, instrumentation and automation, and informatics, and the increased availability of arrayed compound libraries.
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· A high-efficiency, high-throughput and quantitative two-color FACS-based screening strategy was developed to support rapid generation of RNA control devices with user-specified regulatory activities. We validated the high-efficiency of our screening strategy by performing a control sort to isolate the original functional
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We discuss the most significant factors that can improve HTS efficiency, including the construction of screening libraries with high diversity and the use of new detection methods to expand the search range and highlight
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· Directed evolution typically requires extensive screening. This work presents an ultrahigh-throughput microfluidic assay, based on a coupled reaction and fluorescence-activated droplet sorting
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Factors Affecting Throughput. Throughput in Performance Testing isn’t just a random number. It depends on a few things: Your Computer Specs: The horsepower of your host computer matters. It’s like asking if your bicycle or a sports car is faster. Software Overhead: How much work your software has to do in the background affects throughput.
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· We developed P-BEST, a method for Pooling-Based Efficient SARS-CoV-2 Testing, which identifies all positive subjects within a set of samples using a single round of testing. Each sample is assigned into multiple pools using a combinatorial pooling strategy based on compressed sensing. We pooled sets of 384 samples into 48 pools,
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Based on the development of automatic devices and rapid assay methods, various high-throughput screening (HTS) strategies have been established for improving the performance of industrial microorganisms. We discuss the most significant factors that can improve HTS efficiency, including the construction of screening libraries with high
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The screen will operate dry, and screens are available with ‘short slot’ openings and an open area of 65%. Assuming than a screening efficiency of 90% is acceptable, the necessary screen area can then be calculated as follows: U = 500 (0.5) = 250 tons/h of material that should pass through the screen.
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Above 12 mm, dry screening is preferred. Below 12 mm, wet screening using a low-pressure water spray is preferred with a water flow rate of 0.8- 1.4 m 3 /t/h. Actual water requirements dependent on application. An Industrial Screen. [image 145-1-1a] An industrial screen in situ. [image 145-1-1b]
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· An ultrahigh-throughput microfluidic assay, based on a coupled reaction and fluorescence-activated droplet sorting, enabling a 960-fold activity improvement of an amine oxidase for a non-natural substrate in a single round. Biocatalysis provides a potentially sustainable means of chemical manufacturing. However, the tailoring of
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· Herein, a high-throughput optical design strategy for high-performance semitransparent organic solar cells (STOSCs) is demonstrated. Guided by simulations for over tens of millions of device configurations, a photonic-crystal-enhanced STOSC with nearly 11% power conversion efficiency and 30% visible light transmittance was achieved.
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· To boost the screening efficiency, we further optimized a series of parameters for the screening, including fluorescence signal stability, induction time, reaction time, and substrate concentration. The fluorescence signals of the FutA (+) cells were stable for more than 1 hour at room temperature, providing a more-than-ample window for
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· Screening efficiency is the degree of accuracy of which undersize material is passed through an opening. The last important production step in aggregates production is screening (classifying), which has to ensure high-quality screening curves of end products in accordance with standards. It is imperative that the process is executed
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:High-Throughput AssaysHigh-Throughput TechnologyPublish Year:20208 · Employing this strategy to optimize expression efficiency, followed by high-throughput purification as described M. et al. Fully automatized high-throughput
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· huge time cost still limits their application in the high-throughput screening of design and discovery pipeline to screen efficient small molecule acceptors for PTB7 -Th-based organic solar
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· Therefore, improving high-throughput screening (HTS) methods for the detection of transgenic cells expressing desired proteins is necessary to advance algae as an efficient system for recombinant protein production.
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· High-throughput screening platforms can identify biocompatible ligands, which can be used as contrast agents with good targeting efficiency and enhanced tumor uptake, leading to reduced signal interference for
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Dive into the research topics of 'High-throughput screening for efficient microbial biotechnology'. Together they form a unique fingerprint. Miniaturization Medicine & Life Sciences 100%
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· Abstract. High-throughput (HT) methodologies are extensively applied in synthetic biology for the rapid enrichment and selection of desired properties from a wide range of genetic diversity. In order to effectively analyze these vast variants, HT tools must offer parallel experiments and compact reaction capabilities to enhance overall throughput.
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· High-throughput screening should be targeted to maximize strain scale-up potential. Bioreactor control measures are discussed as context for model system design. Sample extractions and assays are compared for throughput and data density. Emergent tools for microfluidic screening of engineered libraries are presented.
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· Therefore, single spore sorting by FC not only improves the efficiency of high-throughput screening but also improves the accuracy of high-throughput screening. Fig. 4 Screening of high α-amylase-producing A. oryzae strains. a Results of the 96-well microplate primary screening combined with FC.
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