: Gea Guerriero, Ian Stokes, Nathalie Valle, Jean-Francois Hausman, Christopher Exley · Silica in plants can be stained by silver Chromate, methyl red, and a colorless crystal violet lactone which are adsorbed by the
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· Silicon is a non-essential element for plants and is available in biota as silicic acid. Its presence has been associated with a general improvement of plant vigour and response to exogenous stresses. Plants accumulate silicon in their tissues as amorphous silica and cell walls are preferential sites. While several papers have been
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A fluorescence resonance energy transfer (FRET) platform for the detection of ozone was developed by combining the overlap of the fluorescence spectrum of Ru(bpy)32+-doped silica nanoparticles with the absorption spectrum of indigo carmine at around 600 nm. This FRET system can be used to detect ozone simply
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· High quality RNA isolated using silica spin column-based method was successfully used for the RT-PCR and/or multiplex RT-PCR amplification of woody fruit tree viruses and a viroid. The study provided a useful tool
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· Owing to incomplete extraction from plant solid matrices, the detection limit of paraquat in plant samples (LOD of 0.1 mg L −1) was higher than that of paraquat in water samples (LOD of 0.05 mg L −1) determined by visualizing the color of AgNPs solution.
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· Silicon (Si) is not considered an essential element, however, its tissue concentration can exceed that of many essential elements in several evolutionary distant plant species. Roots take up
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· To assay for viruses in plant samples, we required a method for nucleic acid isolation that is rapid, simple, and applicable to the widest possible variety of plants. A protocol for isolation of total nucleic acid (TNA) was developed by combining common CTAB methods with silica spin columns. We report data on TNA purity and RNA quality from
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· Silica bodies on plant leaves are colorless and transparent because they are composed of amorphous silicon dioxide (SiO 2 ⋅ n H 2 O) (Piperno, 1988). These properties, together with their small size, mean that may easily be neglected by researchers. However, the silica bodies of Selaginella had been noticed long before modern microscopy.
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· Meanwhile, Ag-carrying mesoporous silica (Ag@MSNs) was prepared as the reinforcement substrate for SERS detection. The results showed that there was a good linear correlation between the Raman intensity and the concentration of chlorpyrifos at 25–250 ng/mL, and the limit of detection (LOD) was 19.87 ng/mL.
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· Elemental silica in water-only treated plants was measured to determine the background contribution of the element and was found to be below the minimum detection level (MDL) (Fig. 11a). We also found MSNs to be co-localized with potassium that is important for normal plant growth (Fig. 11 b).
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Detection of silica in plants. P. Dayanandan, P. Kaufman, C. I. Franklin. Published 1 August 1983. Biology, Environmental Science. American Journal of Botany. Silica in plants can
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It is demonstrated that the fluorescence silica nanoprobe biomarker will have been potential for rapid diagnosis applications on plant diseases. Fluorescent silica nanoprobe as a biomarker for detection has attracted much attention in the field of nano-biotechnology recently but no further research applications using fluorescent silica nanoparticles
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· The rapidly rising population is calling for the production of more food, yet biotic and abiotic stresses caused by pests and climate change substantially diminish crop yields. This issue may be partly solved by applying silica nanoparticles. Nanoparticles are small-sized particles ranging from 1 to 100 nm that possess enhanced physicochemical
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· Fluorescent probes of silica (SiO 2 ) nanoparticles have been used for the detection of a plant pathogen Xanthomonas axonopodis, which is responsible for the bacterial spot disease in plants of
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· While providing excellent detection limits in simple matrices in the low µmol L −1 or even nmol L −1 range 6,21, the detection of the colored silicomolybdic acid complexes 15,16,17,22 can
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· Fluorescent silica nanoparticles coupled with a secondary antibody and doped with a Ru (II) complex have been demonstrated to effectively detect plant pathogens such as X. campestris that cause bacterial spot disease in nightshade plants (Yao et
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· Selaginellaceae (Lycopodiophyta), an early divergent group of vascular plants, are a species-rich and taxonomically challenging family. Here we study silica bodies on the leaves of a prominently studied Selaginella, S. erythropus, with various methods of microscopy and energy disperse X-ray analysis (EDX) to characterize their properties in
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Nanobiosensor-based technology provided a new dimension in plant diseases diagnostic systems (Table 9.2) by offering economical, user-friendly, nondestructive, and minimally invasive system with enhanced sensitivity, specificity, and detection limit for on-the-spot detection of crop pathogens.
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· A recompilation of applications of mesoporous silica nanoparticles in sensing from the last five years is presented. Its high potential, especially as hybrid materials combined with organic or bio-molecules, is shown. Adding to the multiplying effect of loading high amounts of the transducer into the pores, the selectivity attained by the interaction
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· Herein, amorphous silica derived from a geothermal power plant was selected as the fluorescent silica nanoparticles (FSiNP) precursor. Fluorescein isothiocyanate (FITC) was used as the fluorophore incorporated into the silica matrix [26]. The dye-doped silicavia
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There is no standardized method to detect silica depositions in plant tissues. Therefore, we compared current methods to reveal their advantages and disadvantages for selecting
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· Abstract. In plants, pathogen attack can induce an immune response known as systemic acquired resistance that protects against a broad spectrum of pathogens. In the search for safer agrochemicals
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There is no standardized method to detect silica depositions in plant tissues. Therefore, we compared current methods to reveal their advantages and disadvantages for selecting
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· When applied exogenously, silica (Si) can have a beneficial impact on plants under biotic stress conditions, as revealed by its recent application in the form of nanoparticles (SiO 2 NPs) to induce pathogen resistance ( El-Shetehy et al. ). This opens up a new window of research into combating the devastating effects of biotic stresses.
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· Laser induced breakdown spectroscopy (LIBS) has been used to perform in situ analysis of major and minor elements present in the different parts of the Bermuda grass (Cynodon dactylon). In situ, point detection/analysis of the elements in plants without any sample preparation has been demonstrated. LIBS spectra of the different parts (leaf
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· Plant exposure to toxic trace elements influences crop performance and productivity. • Silica nanoparticles have great potential in alleviating metal stress in plants. • Nano-silica is more efficient in its beneficial roles than bulk form of Si. •
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Early online detection of low level silica. When system efficiency is paramount, silica monitoring is a critical measurement. Impurities, like reactive silica, can coat internal process components, leading to decreased efficiency, diminished safety, damage, and costly downtime. Continuous, online measurement of reactive silica is key to
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· Silicon also has been determined gravimetrically in plant tissue as the residue after acid digestion. We have developed a simple inexpensive, and rapid method for solublizing Si in plant tissue that facilitates analysis of a large number of samples.
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· The diluted filtrate from the plant digestion analysed by ICP-OES presented an Si concentration below the detection limit. A simple calculation using the detection limit as a minimum concentration indicated that the quantity of Si present in the filtrate amounted to a maximum 0.0006% of the total plant DW or 0.004% of the phytolith
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· Silicon (Si) is the most copious element of existence in the lithosphere but still it has not been added into the essential element list. The imperative role of Si in triggering growth and development of plants has been identified. It is of paramount importance in regulating overall physiological and metabolic characteristics of the plants.
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